CY5.5 on DNA/RNA probes Cyanine5.5 In situ hybridization FISH

Cyanine 5.5 (Cy5.5) is used in DNA/RNA probes primarily to detect and visualize the presence and distribution of DNA or RNA sequences. Its longer wavelength and higher fluorescence stability make it ideal for DNA/RNA probes. The following are applications of Cy5.5 in DNA/RNA probes:

1. In situ hybridization:

Labeling Cy5.5 on a DNA or RNA probe can be used in in situ hybridization experiments for the detection of specific DNA or RNA sequences. For example, by labeling Cy5.5 on a segment of a DNA probe complementary to a target sequence, the presence and distribution of the target sequence can be observed by in situ hybridization experiments. This application can be used for gene expression analysis, genomic localization and cellular nucleic acid localization.

2. Fluorescence in situ hybridization (FISH):

Labeling Cy5.5 on a DNA or RNA probe can be used in fluorescence in situ hybridization experiments. For example, by labeling Cy5.5 on a segment of DNA probe complementary to the target RNA sequence, the presence and distribution of the target RNA can be directly observed by fluorescence in situ hybridization experiments. This application can be used to study gene expression regulation, cellular nucleic acid localization and chromosome structure.

3. Genome-wide screening:

Labeling Cy5.5 on genome-wide primers can be used in genome-wide screening experiments. For example, labeling Cy5.5 on a set of primers, amplifying DNA from all genomes by PCR, and performing fluorescence detection can identify specific genomic sequences. This application can be used for genome analysis, SNP detection and locus screening.

4. RNA interference (RNAi):

Labeling Cy5.5 on RNAi primers can be used for RNA interference experiments. For example, labeling Cy5.5 on siRNA or shRNA can specifically inhibit the expression of target genes by RNA interference technology, and be detected and verified by the fluorescence signal of Cy5.5. This application can be used for gene function research, gene regulation and disease treatment.

In summary, Cyanine5.5 can be used in DNA/RNA probes for experiments such as in situ hybridization, fluorescence in situ hybridization, whole genome screening and RNA interference. By labeling Cy5.5 on DNA or RNA probes, detection and visualization of specific gene sequences or RNA molecules can be achieved. Such applications can help researchers understand gene expression, genome structure and cellular nucleic acid localization. By combining different experimental techniques and analytical methods, localization, screening and functional studies of specific gene or RNA sequences can be achieved.

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