Application of Cyanine5.5 in immunofluorescence technique CY5.5 multicolor labeling

Cyanine 5.5 (Cy5.5) is widely used in immunofluorescence techniques for antibody labeling and immunohistochemistry. Its longer wavelength and higher fluorescence stability make it ideal for immunofluorescence labeling. The following are applications of Cy5.5 in immunofluorescence techniques:

1. Immunohistochemical labeling:

Labeling Cy5.5 on an antibody can be used for immunohistochemical labeling. For example, labeling Cy5.5 on a specific antibody can be used to detect the distribution of a specific antigen in a cell or tissue. This application can be used to study cell types, tissue structure, and molecular localization, among other things.

2. Multi-color markings:

Cy5.5 can be used in conjunction with other fluorescent dyes (e.g. Cy3, FITC, etc.) to achieve multicolor labeling. For example, antibodies conjugated to Cy5.5 and Cy3 can be used to label different antigens separately, allowing for the simultaneous detection of the distribution of multiple antigens in a sample. This application can be used for multiplex immunohistochemistry, protein interaction studies, etc.

3. Cell surface labeling:

Labeling Cy5.5 on an antibody can be used for cell surface labeling. For example, labeling Cy5.5 on a CD45 antibody can be used for surface labeling for detection of white blood cells. This application can be used for cell phenotyping, immune cell detection, etc.

4. Fluorescence microscopy imaging:

The use of Cy5.5-labeled antibodies can be combined with fluorescence microscopy for observing fluorescent signals from cells or tissues. For example, fluorescence microscopy using the Cy5.5-labeled antibody on an immunotissue section can result in fluorescence images of cells or tissues with high resolution.

In summary, Cyanine5.5 can be used in immunofluorescence techniques for antibody labeling and fluorescence microscopy imaging. By labeling Cy5.5 on specific antibodies, immunohistochemical labeling and detection of specific antigens can be achieved. In addition, Cy5.5 can be used in conjunction with other fluorescent dyes to achieve multicolor labeling. By combining with fluorescence microscopy, fluorescent signals from cells or tissues can be observed, helping researchers to understand cell types, tissue structure and molecular localization.

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